Journal: International Journal of Molecular Sciences

Article Title: Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet

doi: 10.3390/ijms22052431

Figure Lengend Snippet: Effects of HF/HS diet and osthol administration on renal function and markers of renal damage. ( A ) Glomerular filtration rate (GFR), proteinuria, urinary nephrin excretion, N-acetyl-β-D-glucosaminidase (NAG) urine activity, urinary neutrophil gelatinase associated-lipocalin (NGAL) urinary excretion, and kidney injury molecule (KIM)-1 renal cortex expression. ( B ) Pro-inflammatory cytokine expression in the renal cortex (IL-1β and IL-6) and apoptosis biomarker expression (BAX and Bcl2). For Western blotting, 3 randomly selected samples per group were analyzed. Results are presented as the mean ± SD and analyzed by one-way ANOVA. Post hoc analysis was performed using Bonferroni’s test. a: p < 0.05 vs. Control; b: p < 0.05 vs. HF/HS; c: p < 0.05 vs. HF/HS + OT30).

Article Snippet: The antibodies used were as follows: fructokinase (Genetex, GTX109591, 1:5000 dilution), kidney injury molecule (KIM-1) (Genetex, GTX85067, 1:3000 dilution), fatty acid synthase (Genetex, GTX 13550, 1:2500 dilution), xanthine oxidase (Santacruz Biotechnology, sc398548 1:1000 dilution), Nox-4 (Novus Biologicals, NB-110-58851, 1:1000 dilution), nephrin (Genetex, GTX 31654, 1:10,000 dilution), IL-1β (Santacruz Biotechnology, sc-1250, 1:1000 dilution), IL-6 (Santacruz Biotecnology, sc-57315, 1:3000 dilution), phospho-AMPK Thr 172 (Cell Signaling, 40H9, 1:2000 dilution), Sirt-1 (Cell Signaling, D739, 1:2500 dilution), SREBP1 (Genetex, 79299, 1:3000 dilution), Nrf2 (Genetex, 103322, 1:1500 dilution), superoxide dismutase (Santacruz Biotechnology, sc101523, 1:1000 dilution), catalase (Santacruz Biotechnology, sc271358, 1:1500 dilution), glutathione peroxidase (Santacruz Biotechnology, sc22145, 1:1000 dilution), β-actin (Genetex, 109639, 1:10,000 dilution), proliferation cell nuclear antigen (PCNA; GTX100539, 1:2000 dilution), and neutrophil gelatinase associated-lipocalin (NGAL, Santacruz Biotechnology, sc-515876, 1:10,000 dilution).

Techniques: Filtration, Activity Assay, Expressing, Biomarker Assay, Western Blot

Journal: Molecular biology reports

Article Title: Annexin-A5 monomer as a membrane repair agent for the treatment of renal ischemia-reperfusion injury.

doi: 10.1007/s11033-024-09606-2

Figure Lengend Snippet: Fig. 3 AnxA5 reduces the expres sion of early renal injury mark ers. The expression of KIM-1 (a) and NGAL (c) in the kidneys was detected by IHC method (scale bars: 100 μm). (b, d) Semi-quan titative analysis of IHC staining of KIM-1 and NGAL measured by Image-Pro Plus 6.0. Data are presented as the means ± SEM. *p < 0.05, **p < 0.01, *** p < 0.001

Article Snippet: The primary antibodies used in this study are listed below, including kidney injury molecule 1 (KIM-1) (NBP1-76701, 1:400, Novus), neutrophil gelatinase-associated lipocalin (NGAL) (ab216462, 1:1000, Abcam).

Techniques: Expressing, Immunohistochemistry